TNF alpha Protéine

N° du produit ABIN1305133

Protéine Recombinant TNF alpha exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 2+ publications.

Aperçu rapide pour TNF alpha Protéine (ABIN1305133)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Pureté: ≥ 95 %

Détail du produit

Marque: BD Pharmingen™

Specificité: Bioassay: Activity Range Tested by MTT/Actinomycin D Cytolysis Assay using L929 indicator cells
Specific Activity: 0.25 - 5.0 x 10^9 Units /mg
ED50: 2 - 40 pg/ml
Observed Dose response: 250 fold
The Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, titrate the reagent to obtain optimal results.

Attributs du produit: Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat TNF was purified by immunoaffinity chromatography and yielded a TNF protein fraction which was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant rat TNF is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: The endotoxin level is ≤ 0.1 ng/µg of TNF protein, as measured in a chromogenic LAL assay.

Information d'application

Indications d'application: ELISA Standard: Recombinant rat TNF is useful as a quantitative standard for measuring rat TNF protein levels in a TNF specific sandwich ELISA with purified TN3-19.12 antibody (Cat. No. 557516) as a capture antibody and biotinylated rabbit anti-mouse/rat TNF antibody (Cat. No. 557432) as the detection antibody. To obtain linear standard curves, doubling dilutions of rat TNF standard from ~10 ng - 0.1 ng/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For measuring rat TNF in serum or plasma the rat TNF OptEIA™ Set (Cat. No. 558535) and rat TNF OptEIA™ Kit (Cat. No. 560479) are specially formulated and recommended. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

Commentaires:

BD Pharmingen™ Recombinant Rat TNF - Reactivity Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Agent conservateur: Azide free

Conseil sur la manipulation: Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

Stock: -80 °C

Stockage commentaire: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Référence

Jäättelä: "Biologic activities and mechanisms of action of tumor necrosis factor-alpha/cachectin." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 724-42, (1991) (PubMed).

Hogan, Vogel: "Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4196-202, (1989) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Sujet: Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome